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WAPL Polyclonal antibody

WAPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16370-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy WAPL Ready-To-Use IHC Kit. WAPL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16370-1-AP targets WAPL in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 WAPL fusion protein Ag9280 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wings apart-like homolog (Drosophila)
别名 WAPL/FOE, FOE, Wings apart-like protein homolog, WAPL cohesin release factor, WAPAL
计算分子量 142 kDa
观测分子量 160-180 kDa
GenBank蛋白编号BC017393
基因名称 WAPL
Gene ID (NCBI) 23063
RRIDAB_2216716
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z5K2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

实验方案

Product Specific Protocols
WB protocol for WAPL antibody 16370-1-APDownload protocol
IHC protocol for WAPL antibody 16370-1-APDownload protocol
IF protocol for WAPL antibody 16370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,ChIP

Nat Genet

WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation.

Authors - Ning Qing Liu
  • KO Validated
humanWB

Sci Adv

RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis.

Authors - Shu Zhang
mouseWB

J Clin Invest

Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.

Authors - Sun Yaping Y
mouseWB

Genome Biol

CTCF and cohesin promote focal detachment of DNA from the nuclear lamina

Authors - Tom van Schaik
humanWB

Curr Biol

Esco1 Acetylates Cohesin via a Mechanism Different from That of Esco2.

Authors - Masashi Minamino
mouseWB,IF

Cell Rep

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse.

Authors - Miguel A Brieño-Enríquez
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