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  • Featured Product
  • KD/KO Validated

TrpRS Polyclonal antibody

TrpRS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 16081-1-AP

Print datasheet

Synonyms

WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS

验证数据展示

  • WB analysis using 16081-1-AP

    WB analysis using 16081-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16081-1-AP

    WB analysis using 16081-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 16081-1-AP

    IHC staining of human ovary tumor using 16081-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WARS antibody included
  • IHC staining of human ovary tumor using 16081-1-AP

    IHC staining of human ovary tumor using 16081-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WARS antibody included
  • WB analysis using 16081-1-AP

    WB analysis using 16081-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16081-1-AP

    WB analysis using 16081-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16081-1-AP

    IP experiment of HepG2 using 16081-1-AP

    IP result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug.

  • IF Staining of HepG2 using 16081-1-AP

    IF Staining of HepG2 using 16081-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using TrpRS antibody (16081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 16081-1-AP

    FC experiment of HepG2 using 16081-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TrpRS Polyclonal antibody (16081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, HepG2 cells, Jurkat cells, JAR cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16081-1-AP targets TrpRS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TrpRS fusion protein Ag9079 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tryptophanyl-tRNA synthetase
Synonyms WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS
Calculated Molecular Weight 471 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC017489
Gene Symbol WARS1
Gene ID (NCBI) 7453
RRIDAB_2273061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TrpRS antibody 16081-1-APDownload protocol
IHC protocol for TrpRS antibody 16081-1-APDownload protocol
IF protocol for TrpRS antibody 16081-1-APDownload protocol
IP protocol for TrpRS antibody 16081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunother Cancer

Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.

Authors - Rui Qin
  • KO Validated
View Article
humanIHC

Heliyon

Diagnostic, prognostic, and immunological roles of CDSN in ovarian cancer

Authors - Huan Zeng
View Article
humanWB

Cell Mol Life Sci

Tryptophanylation of insulin receptor by WARS attenuates insulin signaling

Authors - Wen-Xing Sun
  • KD Validated
View Article