• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WARS2 Polyclonal antibody

WARS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13944-1-AP

Print datasheet

Synonyms

Tryptophanyl-tRNA synthetase, Tryptophan--tRNA ligase, mitochondrial, Tryptophan tRNA ligase, TrpRS, EC:6.1.1.2

验证数据展示

  • WB analysis of human liver using 13944-1-AP

    WB analysis of human liver using 13944-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13944-1-AP

    IHC staining of human stomach using 13944-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13944-1-AP (WARS2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman liver tissue, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13944-1-AP targets WARS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, pig
Immunogen WARS2 fusion protein Ag5024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tryptophanyl tRNA synthetase 2, mitochondrial
Synonyms Tryptophanyl-tRNA synthetase, Tryptophan--tRNA ligase, mitochondrial, Tryptophan tRNA ligase, TrpRS, EC:6.1.1.2
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 60 kDa/55 kDa
GenBank Accession NumberBC044575
Gene Symbol WARS2
Gene ID (NCBI) 10352
RRIDAB_2304541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WARS2(tryptophan--tRNA ligase, mitochondrial) is also called mitochondrial TRPRS and belongs to the class-I aminoacyl-tRNA synthetase family. The gene encodes the protein containing 360-amino acid ,which has an N-terminal mitochondrial targeting signal that is cleaved after residue 18. WARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold(PMID:15779907). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for WARS2 antibody 13944-1-APDownload protocol
IHC protocol for WARS2 antibody 13944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,pigWB

Virol Sin

Pseudorabies virus manipulates mitochondrial tryptophanyl-tRNA synthetase 2 for viral replication

Authors - Xiu-Qing Li
  • KD Validated
View Article