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- KD/KO Validated
WASP Polyclonal antibody
WASP Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells |
| Positive IP detected in | Raji cells, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy WAS Ready-To-Use IHC Kit. WAS primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IP, RIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | WASP fusion protein Ag1444 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Wiskott-Aldrich syndrome (eczema-thrombocytopenia) |
| Synonyms | Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 55-62 kDa |
| GenBank Accession Number | BC012738 |
| Gene Symbol | WASP |
| Gene ID (NCBI) | 7454 |
| RRID | AB_2304546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WASP antibody 10987-1-AP | Download protocol |
| IHC protocol for WASP antibody 10987-1-AP | Download protocol |
| IP protocol for WASP antibody 10987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. | ||
Nat Commun WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage. | ||
J Exp Clin Cancer Res miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.
| ||
J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. | ||
Mol Cancer Res LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation |
