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WASF4-Specific Polyclonal antibody

WASF4-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19755-1-AP

Print datasheet

Synonyms

SCAR2, WASF4, WAS protein family, member 4

验证数据展示

  • WB analysis of mouse brain using 19755-1-AP

    WB analysis of mouse brain using 19755-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19755-1-AP

    IF Staining of HepG2 using 19755-1-AP

    Immunofluorescent analysis of HepG2 cells, using WASF4 antibody 19755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19755-1-AP targets WASF4-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WAS protein family, member 4
Synonyms SCAR2, WASF4, WAS protein family, member 4
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberQ8IV90
Gene Symbol WASF4
Gene ID (NCBI) 644739
RRIDAB_10646483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF4, also named as SCAR2, belongs to the SCAR/WAVE family. WASF4 is a downstream effector molecule which involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. The antibody is specific to WASF4.

实验方案

Product Specific Protocols
WB protocol for WASF4-Specific antibody 19755-1-APDownload protocol
IF protocol for WASF4-Specific antibody 19755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols