WBP2NL Polyclonal antibody
WBP2NL Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 4 publications below |
产品信息
22587-1-AP targets WBP2NL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | WBP2NL fusion protein Ag18347 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | WBP2 N-terminal like |
| Synonyms | PAWP, WBP2 N terminal like, WBP2NL |
| Calculated Molecular Weight | 309 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC022546 |
| Gene Symbol | WBP2NL |
| Gene ID (NCBI) | 164684 |
| RRID | AB_2879128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ICG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WBP2NL antibody 22587-1-AP | Download protocol |
| IHC protocol for WBP2NL antibody 22587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW binding protein PAWP. | ||
Int J Mol Sci Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers. | ||
Development PLCζ is the physiological trigger of the Ca(2+) oscillations that induce embryogenesis in mammals but offspring can be conceived in its absence. | ||
Mol Hum Reprod Functional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation. | ||
Mol Hum Reprod Antigen unmasking enhances visualization efficacy of the oocyte activation factor, phospholipase C zeta, in mammalian sperm. |