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验证数据展示

  • WB analysis using 13960-1-AP

    WB analysis using 13960-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 13960-1-AP (WDFY1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

WDFY1 Polyclonal antibody

WDFY1 Polyclonal Antibody for WB, ELISA
Cat No. 13960-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

WD repeat and FYVE domain-containing protein 1, Phosphoinositide-binding protein 1, KIAA1435, FYVE domain-containing protein localized to endosomes 1, FENS-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, mouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13960-1-AP targets WDFY1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WDFY1 fusion protein Ag4877 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and FYVE domain containing 1
别名 WD repeat and FYVE domain-containing protein 1, Phosphoinositide-binding protein 1, KIAA1435, FYVE domain-containing protein localized to endosomes 1, FENS-1
计算分子量 410 aa, 46 kDa
观测分子量43-46 kDa
GenBank蛋白编号BC040525
基因名称 WDFY1
Gene ID (NCBI) 57590
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD repeating domain FYVE1 (WDFY1) belongs to the WD40 protein family, which plays key roles in diverse cellular processes, including signal transduction, gene transcription, vesicle fusion, and spermatogenesis(PMID: 32867693; PMID: 10322433). WDFY1 is highly expressed in mouse testis and is dispensable for mouse spermatogenesis and male fertility (PMID: 35121371). The bands of 24 kDa and 19 kDa may be potential isoforms of WDFY1.

实验方案

Product Specific Protocols
WB protocol for WDFY1 antibody 13960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols