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验证数据展示

  • IHC staining of human breast cancer using 25134-1-AP

    IHC staining of human breast cancer using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:400 (under 10x lens).

  • IHC staining of human breast cancer using 25134-1-AP

    IHC staining of human breast cancer using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:400 (under 40x lens).

WDFY3 / ALFY Polyclonal antibody

WDFY3 / ALFY Polyclonal Antibody for IHC, ELISA
Cat No. 25134-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 900 μg/ml

库存：现货

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25134-1-AP targets WDFY3 / ALFY in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen WDFY3 / ALFY fusion protein Ag18393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat and FYVE domain containing 3
Synonyms ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25
Calculated Molecular Weight 3526 aa, 395 kDa
GenBank Accession NumberBC013377
Gene Symbol WDFY3
Gene ID (NCBI) 23001
RRIDAB_2879916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY3 also called ALFY, is a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy.This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.

实验方案

Product Specific Protocols
IHC protocol for WDFY3 / ALFY antibody 25134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols