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验证数据展示

  • IHC staining of rat brain using 55009-1-AP

    IHC staining of rat brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

WDFY3 Polyclonal antibody

WDFY3 Polyclonal Antibody for IHC, ELISA
Cat No. 55009-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA and More (2)

Synonyms

ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55009-1-AP targets WDFY3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat and FYVE domain containing 3
Synonyms ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25
Calculated Molecular Weight 395 kDa
GenBank Accession NumberNM_014991
Gene Symbol WDFY3
Gene ID (NCBI) 23001
RRIDAB_10863126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

实验方案

Product Specific Protocols
IHC protocol for WDFY3 antibody 55009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Cardiovasc Res

WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.

Authors - Shasha Zhang
  • KO Validated
View Article
mouseIHC, IF

In Vitro Cell Dev Biol Anim

Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).

Authors - Huihui Han
View Article