验证数据展示
经过测试的应用
Positive IHC detected in | rat brain tissue, mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55009-1-AP targets WDFY3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | WD repeat and FYVE domain containing 3 |
别名 | ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25 |
计算分子量 | 395 kDa |
GenBank蛋白编号 | NM_014991 |
基因名称 | WDFY3 |
Gene ID (NCBI) | 23001 |
RRID | AB_10863126 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IZQ1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.
实验方案
Product Specific Protocols | |
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IHC protocol for WDFY3 antibody 55009-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cardiovasc Res WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.
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In Vitro Cell Dev Biol Anim Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). |