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WDR16 Polyclonal antibody

WDR16 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12644-1-AP

Print datasheet

Synonyms

WDRPUH, WD40-repeat protein up-regulated in HCC, WD repeat-containing protein 16, WD repeat domain 16, Cilia- and flagella-associated protein 52

验证数据展示

  • IHC staining of human testis using 12644-1-AP

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12644-1-AP

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12644-1-AP

    IF Staining of HeLa using 12644-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12644-1-AP (WDR16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12644-1-AP targets WDR16 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen WDR16 fusion protein Ag3281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 16
Synonyms WDRPUH, WD40-repeat protein up-regulated in HCC, WD repeat-containing protein 16, WD repeat domain 16, Cilia- and flagella-associated protein 52
Calculated Molecular Weight 620 aa, 68 kDa
GenBank Accession NumberBC025392
Gene Symbol WDR16
Gene ID (NCBI) 146845
RRIDAB_2272921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for WDR16 antibody 12644-1-APDownload protocol
IF protocol for WDR16 antibody 12644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols