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验证数据展示

  • IF Staining of MDCK using 26701-1-AP

    IF Staining of MDCK using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of hTERT-RPE1 using 26701-1-AP

    IF Staining of hTERT-RPE1 using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

WDR35 Polyclonal antibody

WDR35 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 26701-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, Canine

应用

IF/ICC, ELISA

别名

IFT121, KIAA1336, WD repeat domain 35, WDR35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells, hTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26701-1-AP targets WDR35 in IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human, Canine
免疫原 WDR35 fusion protein Ag24965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 35
别名 IFT121, KIAA1336, WD repeat domain 35, WDR35
计算分子量 1170 aa, 132 kDa
GenBank蛋白编号BC036659
基因名称 WDR35
Gene ID (NCBI) 57539
RRIDAB_3085893
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2L0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR35, a component of the IFT complex A (IFT-A), is required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). It is involved in ciliogenesis and ciliary protein trafficking (PMID:21473986, PMID:28400947, PMID:29220510). It may promote CASP3 activation and TNF-stimulated apoptosis (PMID: 20193664).

实验方案

Product Specific Protocols
IF protocol for WDR35 antibody 26701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols