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WDR40A Polyclonal antibody

WDR40A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20478-1-AP

Print datasheet

Synonyms

Testis cancer centrosome-related protein, TCC52, KIAA1892, DDB1- and CUL4-associated factor 12, DCAF12

验证数据展示

  • WB analysis of HEK-293 using 20478-1-AP

    WB analysis of HEK-293 using 20478-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 20478-1-AP

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCAF12 antibody included
  • IHC staining of human thyroid cancer using 20478-1-AP

    IHC staining of human thyroid cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCAF12 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DCAF12 Ready-To-Use IHC Kit. DCAF12 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20478-1-AP targets WDR40A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen WDR40A fusion protein Ag14349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 40A
Synonyms Testis cancer centrosome-related protein, TCC52, KIAA1892, DDB1- and CUL4-associated factor 12, DCAF12
Calculated Molecular Weight 453 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008893
Gene Symbol WDR40A
Gene ID (NCBI) 25853
RRIDAB_2878692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T6F0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR40A, also known as DCAF12, is a 51 kDa protein. It may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex(PMID: 16949367). This antibody specifically recognises the 51 kDa protein.

实验方案

Product Specific Protocols
WB protocol for WDR40A antibody 20478-1-APDownload protocol
IHC protocol for WDR40A antibody 20478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols