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验证数据展示

  • WB analysis of HEK-293 using 20478-1-AP

    WB analysis of HEK-293 using 20478-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 20478-1-AP

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCAF12 antibody included
  • IHC staining of human thyroid cancer using 20478-1-AP

    IHC staining of human thyroid cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCAF12 antibody included

WDR40A Polyclonal antibody

WDR40A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20478-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Testis cancer centrosome-related protein, TCC52, KIAA1892, DDB1- and CUL4-associated factor 12, DCAF12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DCAF12 Ready-To-Use IHC Kit. DCAF12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20478-1-AP targets WDR40A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 WDR40A fusion protein Ag14349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 40A
别名 Testis cancer centrosome-related protein, TCC52, KIAA1892, DDB1- and CUL4-associated factor 12, DCAF12
计算分子量 453 aa, 51 kDa
观测分子量 50 kDa
GenBank蛋白编号BC008893
基因名称 WDR40A
Gene ID (NCBI) 25853
RRIDAB_2878692
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T6F0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR40A, also known as DCAF12, is a 51 kDa protein. It may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex(PMID: 16949367). This antibody specifically recognises the 51 kDa protein.

实验方案

Product Specific Protocols
WB protocol for WDR40A antibody 20478-1-APDownload protocol
IHC protocol for WDR40A antibody 20478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols