• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse skeletal muscle using 19194-1-AP (same clone as 19194-1-PBS)

    WB analysis of mouse skeletal muscle using 19194-1-AP (same clone as 19194-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • WB analysis using 19194-1-AP (same clone as 19194-1-PBS)

    WB analysis using 19194-1-AP (same clone as 19194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

    IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WDR45/WIPI4 antibody (19194-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WDR45/WIPI4 Polyclonal antibody, PBS Only

WDR45/WIPI4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19194-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

19194-1-PBS targets WDR45/WIPI4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WDR45/WIPI4 fusion protein Ag6741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 45
别名 WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC000464
基因名称 WDR45
Gene ID (NCBI) 11152
RRIDAB_2215404
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y484
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).