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验证数据展示

  • IHC staining of mouse testis using 23404-1-AP

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 23404-1-AP

    IF Staining of hTERT-RPE1 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 23404-1-AP

    IHC staining of mouse testis using 23404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23404-1-AP (WDR62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 23404-1-AP

    IF Staining of A549 using 23404-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using WDR62 antibody (23404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293T using 23404-1-AP

    IP experiment of HEK-293T using 23404-1-AP

    IP result of anti-WDR62 (IP:23404-1-AP, 4ug; Detection:23404-1-AP 1:500) with HEK-293T cells lysate 1320 ug.

WDR62 Polyclonal antibody

WDR62 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23404-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IP, ELISA

别名

WD repeat-containing protein 62, WD repeat domain 62, C19orf14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHEK-293T cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, A549 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23404-1-AP targets WDR62 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 WDR62 fusion protein Ag19865 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 62
别名 WD repeat-containing protein 62, WD repeat domain 62, C19orf14
计算分子量 1518 aa, 166 kDa
GenBank蛋白编号BC017261
基因名称 WDR62
Gene ID (NCBI) 284403
RRIDAB_3085711
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571).

实验方案

Product Specific Protocols
IHC protocol for WDR62 antibody 23404-1-APDownload protocol
IF protocol for WDR62 antibody 23404-1-APDownload protocol
IP protocol for WDR62 antibody 23404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols