WDR7 Polyclonal antibody
WDR7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
24431-1-AP targets WDR7 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, rat, mouse |
| Immunogen | WDR7 fusion protein Ag19072 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | WD repeat domain 7 |
| Synonyms | KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7 |
| Calculated Molecular Weight | 1490 aa, 164 kDa |
| Observed Molecular Weight | 145-150 kDa |
| GenBank Accession Number | BC131558 |
| Gene Symbol | WDR7 |
| Gene ID (NCBI) | 23335 |
| RRID | AB_2879546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4E6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR7 antibody 24431-1-AP | Download protocol |
| IHC protocol for WDR7 antibody 24431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
