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验证数据展示

  • WB analysis using 24431-1-AP

    WB analysis using 24431-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24431-1-AP (WDR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24431-1-AP

    IHC staining of human liver using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 24431-1-AP

    IHC staining of human liver using 24431-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24431-1-AP (WDR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WDR7 Polyclonal antibody

WDR7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24431-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA and More (1)

别名

KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

24431-1-AP targets WDR7 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, rat, mouse
免疫原 WDR7 fusion protein Ag19072 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 7
别名 KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7
计算分子量 1490 aa, 164 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC131558
基因名称 WDR7
Gene ID (NCBI) 23335
RRIDAB_2879546
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4E6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.

实验方案

Product Specific Protocols
WB protocol for WDR7 antibody 24431-1-APDownload protocol
IHC protocol for WDR7 antibody 24431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

J Biol Chem

The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.

Authors - Ellen Crummy
View Article