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WDR77 Polyclonal antibody

WDR77 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10115-1-AP

Print datasheet

Synonyms

WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77

验证数据展示

  • WB analysis using 10115-1-AP

    WB analysis using 10115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10115-1-AP (WDR77 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 10115-1-AP

    IHC staining of human stomach using 10115-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10115-1-AP (WDR77 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10115-1-AP targets WDR77 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen WDR77 fusion protein Ag0147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 77
Synonyms WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77
Calculated Molecular Weight 50 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC001679
Gene Symbol WDR77
Gene ID (NCBI) 79084
RRIDAB_2215725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.

实验方案

Product Specific Protocols
WB protocol for WDR77 antibody 10115-1-APDownload protocol
IHC protocol for WDR77 antibody 10115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression

Authors - Yan Sun
View Article