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验证数据展示

  • WB analysis using 10115-1-AP

    WB analysis using 10115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10115-1-AP (WDR77 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 10115-1-AP

    IHC staining of human stomach using 10115-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10115-1-AP (WDR77 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WDR77 Polyclonal antibody

WDR77 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10115-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10115-1-AP targets WDR77 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 WDR77 fusion protein Ag0147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 77
别名 WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77
计算分子量 50 kDa
观测分子量40 kDa
GenBank蛋白编号BC001679
基因名称 WDR77
Gene ID (NCBI) 79084
RRIDAB_2215725
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.

实验方案

Product Specific Protocols
WB protocol for WDR77 antibody 10115-1-APDownload protocol
IHC protocol for WDR77 antibody 10115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression

Authors - Yan Sun
View Article