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  • Featured Product
  • KD/KO Validated

WDR81 Polyclonal antibody

WDR81 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24874-1-AP

Print datasheet

Synonyms

WD repeat-containing protein 81

验证数据展示

  • WB analysis using 24874-1-AP

    WB analysis using 24874-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 24874-1-AP

    IHC staining of mouse cerebellum using 24874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 24874-1-AP

    IHC staining of mouse cerebellum using 24874-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 24874-1-AP

    IF Staining of mouse cerebellum using 24874-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using WDR81 antibody (24874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 24874-1-AP

    IF Staining of HepG2 using 24874-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR81 antibody (24874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, SH-SY5Y cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24874-1-AP targets WDR81 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen WDR81 fusion protein Ag21666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 81
Synonyms WD repeat-containing protein 81
Calculated Molecular Weight 1941 aa, 212 kDa
Observed Molecular Weight74-80 kDa, 250 kDa
GenBank Accession NumberBC114568
Gene Symbol WDR81
Gene ID (NCBI) 124997
RRIDAB_2879771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ562E7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.

实验方案

Product Specific Protocols
WB protocol for WDR81 antibody 24874-1-APDownload protocol
IHC protocol for WDR81 antibody 24874-1-APDownload protocol
IF protocol for WDR81 antibody 24874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Mol Psychiatry

WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.

Authors - Min Wang
  • KO Validated
View Article
humanWB

Neurogenetics

Fetal brain arrest broadens the spectrum of WDR81-related developmental brain malformations.

Authors - Mohamed S Abdel-Hamid
View Article