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WEE1 Polyclonal antibody, PBS Only

WEE1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29474-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29474-1-PBS in a different storage buffer formulation.

产品信息

29474-1-PBS targets WEE1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen WEE1 fusion protein Ag30194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WEE1 homolog (S. pombe)
Synonyms
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa,110 kDa
GenBank Accession NumberBC051831
Gene Symbol WEE1
Gene ID (NCBI) 7465
RRIDAB_2918314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30291
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).