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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    WB analysis using 29474-1-AP (same clone as 29474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29474-1-PBS in a different storage buffer formulation.

WEE1 Polyclonal antibody, PBS Only

WEE1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29474-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

29474-1-PBS targets WEE1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 WEE1 fusion protein Ag30194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WEE1 homolog (S. pombe)
别名
计算分子量 72 kDa
观测分子量72 kDa,110 kDa
GenBank蛋白编号BC051831
基因名称 WEE1
Gene ID (NCBI) 7465
RRIDAB_2918314
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30291
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).