• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WIBG Polyclonal antibody

WIBG Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14658-1-AP

Print datasheet

Synonyms

PYM1, PYM homolog 1 exon junction complex-associated factor, PYM, Protein wibg homolog, Partner of Y14 and mago

验证数据展示

  • IHC staining of mouse colon using 14658-1-AP

    IHC staining of mouse colon using 14658-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14658-1-AP (WIBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 14658-1-AP

    IF Staining of U2OS using 14658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using WIBG antibody (14658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14658-1-AP targets WIBG in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen WIBG fusion protein Ag6276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name within bgcn homolog (Drosophila)
Synonyms PYM1, PYM homolog 1 exon junction complex-associated factor, PYM, Protein wibg homolog, Partner of Y14 and mago
Calculated Molecular Weight 23 kDa
GenBank Accession NumberBC006135
Gene Symbol WIBG
Gene ID (NCBI) 84305
RRIDAB_3085439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein WIBG homolog, also known as PYM1 (Partner of Y14 and mago), is a cytoplasmic RNA-binding protein, that can interact with the ribosome and EJC, may act as an EJC destabilizing factor, preventing EJC accumulation at positions away from exon-exon junctions (non-canonical position).

实验方案

Product Specific Protocols
IHC protocol for WIBG antibody 14658-1-APDownload protocol
IF protocol for WIBG antibody 14658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols