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WIPF1 Polyclonal antibody

WIPF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22047-1-AP

Print datasheet

Synonyms

Protein PRPL 2, PRPL 2, WASP interacting protein, WASPIP, WIP, WIPF1

验证数据展示

  • WB analysis using 22047-1-AP

    WB analysis using 22047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22047-1-AP (WIPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 22047-1-AP

    IHC staining of human stomach cancer using 22047-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22047-1-AP (WIPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22047-1-AP targets WIPF1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen WIPF1 fusion protein Ag16975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WAS/WASL interacting protein family, member 1
Synonyms Protein PRPL 2, PRPL 2, WASP interacting protein, WASPIP, WIP, WIPF1
Calculated Molecular Weight403aa,40 kDa; 503aa,51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC110288
Gene Symbol WIPF1
Gene ID (NCBI) 7456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43516
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429).

实验方案

Product Specific Protocols
WB protocol for WIPF1 antibody 22047-1-APDownload protocol
IHC protocol for WIPF1 antibody 22047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols