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验证数据展示

  • IHC staining of human breast cancer using 20512-1-AP

    IHC staining of human breast cancer using 20512-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with WIPF2 antibody included
  • WB analysis of mouse brain using 20512-1-AP

    WB analysis of mouse brain using 20512-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WIPF2 Polyclonal antibody

WIPF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20512-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

WICH, WIP related protein, WIPF2, WIRE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse colon tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WIPF2 Ready-To-Use IHC Kit. WIPF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20512-1-AP targets WIPF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WIPF2 fusion protein Ag14330 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS/WASL interacting protein family, member 2
别名 WICH, WIP related protein, WIPF2, WIRE
计算分子量 440 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC065551
基因名称 WIPF2
Gene ID (NCBI) 147179
RRIDAB_10695185
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TF74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WIPF2 antibody 20512-1-APDownload protocol
IHC protocol for WIPF2 antibody 20512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols