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验证数据展示

  • WB analysis of Caco-2 using 21259-1-AP

    WB analysis of Caco-2 using 21259-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 21259-1-AP (WISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 21259-1-AP

    IHC staining of human colon cancer using 21259-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 21259-1-AP

    IHC staining of human colon cancer using 21259-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21259-1-AP (WISP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WISP3 Polyclonal antibody

WISP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21259-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CCN family member 6, CCN6, LIBC, PPAC, PPD, WISP 3, WISP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21259-1-AP targets WISP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen WISP3 fusion protein Ag15238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WNT1 inducible signaling pathway protein 3
Synonyms CCN family member 6, CCN6, LIBC, PPAC, PPD, WISP 3, WISP3
Calculated Molecular Weight 372 aa, 41 kDa
Observed Molecular Weight39-41 kDa
GenBank Accession NumberBC012028
Gene Symbol WISP3
Gene ID (NCBI) 8838
RRIDAB_2918070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95389
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WISP3 antibody 21259-1-APDownload protocol
IHC protocol for WISP3 antibody 21259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients

Authors - Liping Shi
View Article
humanWB

Exp Hematol Oncol

Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway

Authors - Fei Zhou
View Article