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验证数据展示

  • IHC staining of human heart using 22297-1-AP

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 22297-1-AP

    IF Staining of HepG2 using 22297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22297-1-AP (WNK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

WNK2 Polyclonal antibody

WNK2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 22297-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22297-1-AP targets WNK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WNK lysine deficient protein kinase 2
别名 Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2
计算分子量 243 kDa
GenBank蛋白编号NM_006648
基因名称 WNK2
Gene ID (NCBI) 65268
RRIDAB_2935457
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3S1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for WNK2 antibody 22297-1-APDownload protocol
IF protocol for WNK2 antibody 22297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols