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验证数据展示

  • IHC staining of human heart using 22297-1-AP

    IHC staining of human heart using 22297-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22297-1-AP (WNK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 22297-1-AP

    IF Staining of HepG2 using 22297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22297-1-AP (WNK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

WNK2 Polyclonal antibody

WNK2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 22297-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22297-1-AP targets WNK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WNK lysine deficient protein kinase 2
Synonyms Antigen NY CO 43, KIAA1760, NY CO 43, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2
Calculated Molecular Weight 243 kDa
GenBank Accession NumberNM_006648
Gene Symbol WNK2
Gene ID (NCBI) 65268
RRIDAB_2935457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3S1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for WNK2 antibody 22297-1-APDownload protocol
IF protocol for WNK2 antibody 22297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols