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WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22326-1-AP

Print datasheet

Synonyms

PHA2B, PRKWNK4, WNK4

验证数据展示

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 22326-1-AP

    WB analysis using 22326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22326-1-AP (WNK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA375 cells, K-562 cells, PC-12 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

22326-1-AP targets WNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen WNK4 fusion protein Ag17773 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WNK lysine deficient protein kinase 4
Synonyms PHA2B, PRKWNK4, WNK4
Calculated Molecular Weight 1243 aa, 135 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC136664
Gene Symbol WNK4
Gene ID (NCBI) 65266
RRIDAB_2879072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96J92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WNK4 antibody 22326-1-APDownload protocol
IHC protocol for WNK4 antibody 22326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan
View Article
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori
View Article
mouseWB

bioRxiv

DOPAMINE D4 RECEPTOR DOWN-REGULATES RENAL SODIUM CHLORIDE COTRANSPORTER VIA UBIQUITINATION-ASSOCIATED LYSOSOME DEGRADATION

Authors - Mingzhuo Zhang
View Article