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验证数据展示

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 22326-1-AP

    WB analysis using 22326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22326-1-AP (WNK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22326-1-AP (WNK4 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22326-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, PRKWNK4, PHA2B, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, K-562 cells, PC-12 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

22326-1-AP targets WNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 WNK4 fusion protein Ag17773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WNK lysine deficient protein kinase 4
别名 Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, PRKWNK4, PHA2B, EC:2.7.11.1
计算分子量 1243 aa, 135 kDa
观测分子量 180 kDa
GenBank蛋白编号BC136664
基因名称 WNK4
Gene ID (NCBI) 65266
RRIDAB_2879072
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WNK4 antibody 22326-1-APDownload protocol
IHC protocol for WNK4 antibody 22326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan
View Article
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori
View Article
mouseWB

bioRxiv

DOPAMINE D4 RECEPTOR DOWN-REGULATES RENAL SODIUM CHLORIDE COTRANSPORTER VIA UBIQUITINATION-ASSOCIATED LYSOSOME DEGRADATION

Authors - Mingzhuo Zhang
View Article