WNT10A Polyclonal antibody

WNT10A Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26238-1-AP

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Synonyms

Protein Wnt 10a, WNT10, WNT10A



经过测试的应用

Positive IHC detected inhuman oesophagus cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26238-1-AP is WNT10A Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, goat
Immunogen WNT10A fusion protein Ag23963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 10A
Synonyms Protein Wnt 10a, WNT10, WNT10A
Calculated Molecular Weight 417 aa, 46 kDa
GenBank Accession NumberBC052234
Gene Symbol WNT10A
Gene ID (NCBI) 80326
RRIDAB_2880439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT10A is a member of the WNT gene family, which consists of structurally related genes encoding secreted signaling molecules critical for many organogenesis processes. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines.

实验方案

Product Specific Protocols
IHC protocol for WNT10A antibody 26238-1-APDownload protocol
IF protocol for WNT10A antibody 26238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Identification of the prognostic value of a 2-gene signature of the WNT gene family in UCEC using bioinformatics and real-world data.

Authors - Yuexin Hu
humanIHC

Oral Dis

Long non-coding RNAs MALAT1 and NEAT1 in non-syndromic orofacial clefts.

Authors - Errui Wang
goatWB,IF

J Anim Sci Biotechnol

Melatonin promotes the development of the secondary hair follicles by regulating circMPP5

Authors - Xiaogao Diao
mouseIHC

Kidney Int

Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis

Authors - Dong Zhou