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验证数据展示

  • WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

    WB analysis using 66656-1-Ig (same clone as 66656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66656-1-Ig (same clone as 66656-1-PBS)

    IF Staining of MCF-7 using 66656-1-Ig (same clone as 66656-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66656-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WNT2 Monoclonal antibody, PBS Only

WNT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66656-1-PBS

产品说明书

CloneNo. 3H7B10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66656-1-PBS targets WNT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 WNT2 fusion protein Ag25185 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family member 2
别名 Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2
计算分子量 40 kDa
观测分子量 36 kDa
GenBank蛋白编号BC029854
基因名称 WNT2
Gene ID (NCBI) 7472
RRIDAB_2882013
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09544
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.