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验证数据展示

  • WB analysis using 28156-1-AP

    WB analysis using 28156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28156-1-AP

    IHC staining of mouse brain using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 28156-1-AP

    IHC staining of mouse colon using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 28156-1-AP

    IHC staining of mouse colon using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28156-1-AP

    IHC staining of mouse kidney using 28156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WNT3 Polyclonal antibody

WNT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, MCF-7 cells
Positive IHC detected inmouse colon tissue, mouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28156-1-AP targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 WNT3 fusion protein Ag27965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 3
别名 INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3
计算分子量 355 aa, 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC114219
基因名称 WNT3
Gene ID (NCBI) 7473
RRIDAB_3669649
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56703
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).

实验方案

Product Specific Protocols
WB protocol for WNT3 antibody 28156-1-APDownload protocol
IHC protocol for WNT3 antibody 28156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols