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WNT7A/B Polyclonal antibody

WNT7A/B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10605-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

WNT7B, WNT7A, Protein Wnt 7a, Protein Wnt-7a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10605-1-AP targets WNT7A/B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 WNT7A/B fusion protein Ag0874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 7A
别名 WNT7B, WNT7A, Protein Wnt 7a, Protein Wnt-7a
计算分子量 39 kDa
观测分子量30-39 kDa
GenBank蛋白编号BC008811
基因名称 WNT7A
Gene ID (NCBI) 7476
RRIDAB_2215625
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00755
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.

实验方案

Product Specific Protocols
WB protocol for WNT7A/B antibody 10605-1-APDownload protocol
IHC protocol for WNT7A/B antibody 10605-1-APDownload protocol
IF protocol for WNT7A/B antibody 10605-1-APDownload protocol
IP protocol for WNT7A/B antibody 10605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Front Oncol

The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.

Authors - Chun Tang
mouseIF

Development

Aberrant centrosome biogenesis disrupts nephron and collecting duct progenitor growth and fate resulting in fibrocystic kidney disease

Authors - Tao Cheng
humanWB,IHC

Oncol Rep

Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma.

Authors - Bo Jia
  • KD Validated
mouseWB

Behav Brain Res

OGDHL ameliorates cognitive impairment and Alzheimer's disease-like pathology via activating Wnt/β-catenin signaling in Alzheimer's disease mice.

Authors - Li Yao
WB

Oncol Rep

RNA-binding motif protein 5 negatively regulates the activity of Wnt/β-catenin signaling in cigarette smoke-induced alveolar epithelial injury.

Authors - Yu-Qiu Hao
mouseWB,IHC

Neurol Res

Wnt signaling pathway is involved in the pathogenesis of amyotrophic lateral sclerosis in adult transgenic mice.

Authors - Chen Yanchun Y
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