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验证数据展示

  • WB analysis using 27177-1-AP

    WB analysis using 27177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 27177-1-AP

    IP experiment of mouse kidney using 27177-1-AP

    IP result of anti-WNT7A (IP:27177-1-AP, 4ug; Detection:27177-1-AP 1:2000) with mouse kidney tissue lysate 2160 ug.

  • IF Staining of HepG2 using 27177-1-AP

    IF Staining of HepG2 using 27177-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

WNT7A Polyclonal antibody

WNT7A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 27177-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

WNT 7A, Protein Wnt-7a, Protein Wnt 7a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

27177-1-AP targets WNT7A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 WNT7A fusion protein Ag26060 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 7A
别名 WNT 7A, Protein Wnt-7a, Protein Wnt 7a
计算分子量 39 kDa
观测分子量 30-39 kDa
GenBank蛋白编号BC008811
基因名称 WNT7A
Gene ID (NCBI) 7476
RRIDAB_3085932
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00755
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).

实验方案

Product Specific Protocols
WB protocol for WNT7A antibody 27177-1-APDownload protocol
IF protocol for WNT7A antibody 27177-1-APDownload protocol
IP protocol for WNT7A antibody 27177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

EMBO Rep

Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes

Authors - Yuan Gao
View Article