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WRN Polyclonal antibody

WRN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25269-1-AP

Print datasheet

Synonyms

DNA helicase, RecQ like type 3, Exonuclease WRN, RecQ protein like 2, RECQ3, RECQL2, RECQL3, WRN

验证数据展示

  • WB analysis of MCF-7 using 25269-1-AP

    WB analysis of MCF-7 using 25269-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25269-1-AP (WRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25269-1-AP targets WRN in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Werner syndrome, RecQ helicase-like
Synonyms DNA helicase, RecQ like type 3, Exonuclease WRN, RecQ protein like 2, RECQ3, RECQL2, RECQL3, WRN
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_000553
Gene Symbol WRN
Gene ID (NCBI) 7486
RRIDAB_2879997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14191
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for WRN antibody 25269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols