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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 11666-1-AP (same clone as 11666-1-PBS)

    WB analysis using 11666-1-AP (same clone as 11666-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11666-1-AP (same clone as 11666-1-PBS)

    IHC staining of human prostate cancer using 11666-1-AP (same clone as 11666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11666-1-AP (WSB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11666-1-AP (same clone as 11666-1-PBS)

    IF Staining of HepG2 using 11666-1-AP (same clone as 11666-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

WSB1 Polyclonal antibody, PBS Only

WSB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11666-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11666-1-PBS targets WSB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 WSB1 fusion protein Ag2211 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and SOCS box-containing 1
别名
计算分子量 421 aa, 47 kDa
观测分子量 56 kDa
GenBank蛋白编号BC021110
基因名称 WSB1
Gene ID (NCBI) 26118
RRIDAB_10949076
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6I7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WSB1 (or SWIP1) gene encodes WD repeat and SOCS box-containing protein 1, a member of the WD-protein subfamily. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding 3 distinct isoforms have been found for this gene. WSB1 serves as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WSB1 has been reported (PMID: 15965468), initiated by the interaction of the two components (PMID: 18093972). WSB1 is also a part of an E3 ubiquitin ligase for the thyroid-hormone-activating type 2 iodothyronine deiodinase (D2) (PMID: 15965468).