验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, T-47D cells, MDA-MB-231 cells, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68361-1-Ig targets WSTF in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | WSTF fusion protein Ag24403 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | bromodomain adjacent to zinc finger domain, 1B |
| 别名 | BAZ1B, hWALp2, Tyrosine protein kinase BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF |
| 计算分子量 | 1483 aa, 171 kDa |
| 观测分子量 | 170 kDa |
| GenBank蛋白编号 | BC136520 |
| 基因名称 | WSTF/BAZ1B |
| Gene ID (NCBI) | 9031 |
| RRID | AB_3085083 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UIG0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tyrosine-protein kinase BAZ1B, also named WSTF, plays a central role in chromatin remodeling and acts as a transcription regulator (PMID:19092802). It involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph) (PMID:19234442).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WSTF antibody 68361-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |