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  • Featured Product
  • KD/KO Validated

WTAP Monoclonal antibody, PBS Only

WTAP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A10G9

Cat no : 60188-1-PBS

Print datasheet

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

验证数据展示

  • WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

    IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WTAP antibody included
  • IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy WTAP Ready-To-Use IHC Kit. WTAP primary antibody included.

产品信息

60188-1-PBS targets WTAP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen WTAP fusion protein Ag0259 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Wilms tumor 1 associated protein
Synonyms Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC000383
Gene Symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15007
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.