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Western Blot 封闭液

Cat No. PR20011

特性

降低背景,增强信噪比

应用

WB

规格价格库存


Product Information

Proteintech 生产的 Western Blot 封闭液适用于 Western Blot 过程中对已转移蛋白样品的 PVDF膜或NC膜进行封闭,可以降低背景,增强信噪比。

产品介绍

组分/规格100 mL
脱脂奶粉5 g
TBST100 mL


保存条件

奶粉室温干燥保存,液体试剂4℃保存,有效期12个月。

使用方法

1. 使用前向 Western Blot 封闭液中加入脱脂奶粉配制成 5%工作液,混匀即可。

2. 将转移后的膜浸入 Western Blot 封闭液,置于摇床,室温 2 小时或 4℃过夜。

3. 封闭结束后去除封闭液,进行 Western Blot后续实验。

4. Western Blot 操作请参考本公司网站:www.ptgcn.com

注意

1. 室温封闭 2 小时或 4℃封闭过夜。

2. 每次使用时,印迹膜必须完全浸没在封闭液中,以确保封闭效果。

3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

4. 配制好的试剂含有牛奶,不建议存放、反复使用,极易变质。

Publications

ApplicationTitle

FASEB J

Sirt1-Mediated Transcriptional Inhibition of Nr4a3 Alleviates Severe Acute Pancreatitis-Associated Acute Lung Injury

Authors - Xiaolin Dou

J Biol Chem

IL4I1 overexpression protects against nonalcoholic fatty liver disease in part by inhibiting the AKT/FOXO1 pathway-mediated Th17 cell differentiation

Authors - Yijun Yang

J Surg Res

Interferon Regulatory Factor1 Deficiency Ameliorates Hemorrhagic Shock-Induced Kidney Injury Via IGFBP7/IGF1R Pathway.

Authors - Jimin Cai

In Vitro Cell Dev Biol Anim

KLF5 drives granulosa cell apoptosis and inflammatory injury in polycystic ovary syndrome via transcriptional activation of TXNIP.

Authors - Ying Wang

Int Immunopharmacol

Periprosthetic osteolysis is mediated by the m6A-dependent regulation of CEMIP via YTHDF2.

Authors - Zeming Lei

Int J Mol Med

DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip‑associated secondary osteoarthritis.

Authors - ENBO WANG
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