Validation Data Gallery
Product Information
Proteintech 生产的 Western Blot 封闭液适用于 Western Blot 过程中对已转移蛋白样品的 PVDF膜或NC膜进行封闭,可以降低背景,增强信噪比。
产品介绍
| 组分/规格 | 100 mL |
| 脱脂奶粉 | 5 g |
| TBST | 100 mL |
保存条件
奶粉室温干燥保存,液体试剂4℃保存,有效期12个月。
使用方法
1. 使用前向 Western Blot 封闭液中加入脱脂奶粉配制成 5%工作液,混匀即可。
2. 将转移后的膜浸入 Western Blot 封闭液,置于摇床,室温 2 小时或 4℃过夜。
3. 封闭结束后去除封闭液,进行 Western Blot后续实验。
4. Western Blot 操作请参考本公司网站:www.ptgcn.com
注意
1. 室温封闭 2 小时或 4℃封闭过夜。
2. 每次使用时,印迹膜必须完全浸没在封闭液中,以确保封闭效果。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
4. 配制好的试剂含有牛奶,不建议存放、反复使用,极易变质。
Publications
| Application | Title |
|---|---|
Cell Death Discov NFATC2-mediated CST1 upregulation drives cholangiocarcinoma growth and metastasis. | |
Int J Mol Med DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip‑associated secondary osteoarthritis. | |
CNS Neurosci Ther Insulin-Like Growth Factor Binding Protein 2 Drives Neurodegeneration in Parkinson's Disease: Insights From In Vivo and In Vitro Studies | |
Biochem Pharmacol BDH2 promotes GLIPR1 promoter methylation and thus increases 5-FU sensitivity in colorectal cancer | |
Biochem Pharmacol Accelerating bone healing in femoral defect model using FBXO6-modified bone marrow-derived mesenchymal stem cells on a collagen scaffold. | |
Biochem Pharmacol SEMA7A orchestrates the colorectal cancer tumor microenvironment via PlexinC1: coordinating angiogenesis and macrophage M2 polarization. |
