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X-NeuNAc

X-NeuNAc (5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) 是细菌表达系统中神经氨酸酶活性的一种新的显色测定底物。

CAS号

160369-85-7

分子式

C19H21BrClN2NaO9

主要靶点

Others

仅限科研使用

Cat No : CM18400

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Synonyms

5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt|X-Neu5Ac

验证数据展示

  • CAS No.: 160369-85-7

    X-NeuNAc
    CAS#: 160369-85-7

产品信息

X-NeuNAc is a new chromogenic assay substrate of neuraminidase activity in bacterial expression systems.

CAS号 160369-85-7
分子式 C19H21BrClN2NaO9
主要靶点 Others
主要通路 其他
分子量 559.72
纯度 100.00%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt|X-Neu5Ac

体内活性

To visualize extracellular sialidase activity on the membrane surface in the rat brain, acute brain slices were incubated with X-Neu5Ac at pH 7.3. After 1h, myelin-abundant regions showed intense fluorescence in the rat brain. Although the hippocampus showed weak fluorescence in the brain, mossy fiber terminals in the hippocampus showed relatively intense fluorescence. In addition, the fluorescence intensities caused by X-Neu5Ac was correlated with the sialidase activity. Therefore, staining with X-Neu5Ac was specific for sialidase and useful for quantitative analysis of sialidase activities [1].

体外活性

X-Neu5Ac was hydrolyzed by neuraminidase to release a halogenated product undergoing rapid aerobic oxidation to form the dark blue pigment. Preliminary kinetic studies indicated that X-Neu5Ac was a good and stable substrate for neuraminidase. In addition, X-Neu5Ac would also be hydrolyzed mutant enzymes

溶解度

DMSO:60 mg/ml (107.2 mM)

参考文献

1.Fujii I,Iwabuchi Y,Teshima T,Shiba T,Kikuchi M.  X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. Bioorg Med Chem.1993 Aug;1(2):147-9.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
=
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2