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XCL1 Polyclonal antibody

XCL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26605-1-AP

Print datasheet

Synonyms

Lymphotactin, Small-inducible cytokine C1, SCM-1-alpha, Cytokine SCM-1, C motif chemokine 1

验证数据展示

  • WB analysis using 26605-1-AP

    WB analysis using 26605-1-AP

    Untreated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 26605-1-AP (Lymphotactin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26605-1-AP

    IHC staining of human tonsillitis using 26605-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26605-1-AP (XCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPMA, Ionomycin and Brefeldin A treated NK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26605-1-AP targets XCL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen XCL1 fusion protein Ag24258 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C motif) ligand 1
Synonyms Lymphotactin, Small-inducible cytokine C1, SCM-1-alpha, Cytokine SCM-1, C motif chemokine 1
Calculated Molecular Weight 114 aa, 13 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC070309
Gene Symbol XCL1
Gene ID (NCBI) 6375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47992
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XCL1 also known as Lymphotactin, is a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation.

实验方案

Product Specific Protocols
WB protocol for XCL1 antibody 26605-1-APDownload protocol
IHC protocol for XCL1 antibody 26605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols