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  • Featured Product
  • KD/KO Validated

XIAP Monoclonal antibody, PBS Only

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A1A12

Cat no : 66800-1-PBS

Print datasheet

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

验证数据展示

  • WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

产品信息

66800-1-PBS targets XIAP in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen XIAP fusion protein Ag18088 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name X-linked inhibitor of apoptosis
Synonyms API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP98170
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.