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  • Featured Product
  • KD/KO Validated

XIAP Monoclonal antibody, PBS Only

XIAP Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

2A1A12

Cat No : 66800-1-PBS

Print datasheet

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

验证数据展示

  • WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

产品信息

66800-1-PBS targets XIAP in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, mouse, rat
Immunogen XIAP fusion protein Ag18088 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name X-linked inhibitor of apoptosis
Synonyms API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC032729
Gene Symbol XIAP
Gene ID (NCBI) 331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP98170
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.