验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, Caco-2 cells, human heart tissue |
| Positive IHC detected in | human skeletal muscle tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11896-1-AP targets XIRP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | XIRP2 fusion protein Ag2515 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | xin actin-binding repeat containing 2 |
| Synonyms | Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin |
| Calculated Molecular Weight | 379 kDa |
| Observed Molecular Weight | 260-300 kDa |
| GenBank Accession Number | BC022888 |
| Gene Symbol | XIRP2 |
| Gene ID (NCBI) | 129446 |
| RRID | AB_10951481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A4UGR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XIRP2 antibody 11896-1-AP | Download protocol |
| IHC protocol for XIRP2 antibody 11896-1-AP | Download protocol |
| IF protocol for XIRP2 antibody 11896-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain | ||
Front Physiol Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling. | ||
Biology (Basel) Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma
|
