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验证数据展示

  • WB analysis of COLO 320 using 11896-1-AP

    WB analysis of COLO 320 using 11896-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 11896-1-AP

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 11896-1-AP

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse heart using 11896-1-AP

    IF Staining of mouse heart using 11896-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

XIRP2 Polyclonal antibody

XIRP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11896-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, Caco-2 cells, human heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11896-1-AP targets XIRP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 XIRP2 fusion protein Ag2515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 xin actin-binding repeat containing 2
别名 Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin
计算分子量 379 kDa
观测分子量 260-300 kDa
GenBank蛋白编号BC022888
基因名称 XIRP2
Gene ID (NCBI) 129446
RRIDAB_10951481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA4UGR9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XIRP2 antibody 11896-1-APDownload protocol
IHC protocol for XIRP2 antibody 11896-1-APDownload protocol
IF protocol for XIRP2 antibody 11896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Elife

Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain

Authors - Elizabeth L Wagner
View Article
mouseWB,IF

Front Physiol

Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.

Authors - Emily Farrell
View Article
humanWB,IF

Biology (Basel)

Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma

Authors - Dahuan Li
  • KD Validated
View Article