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XK Polyclonal antibody

XK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12830-1-AP

Print datasheet

Synonyms

Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1

验证数据展示

  • WB analysis of mouse pancreas using 12830-1-AP

    WB analysis of mouse pancreas using 12830-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12830-1-AP

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas cancer using 12830-1-AP

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse pancreas tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12830-1-AP targets XK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen XK fusion protein Ag3550 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-linked Kx blood group (McLeod syndrome)
Synonyms Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1
Calculated Molecular Weight 444 aa, 51 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberBC036019
Gene Symbol XK
Gene ID (NCBI) 7504
RRIDAB_2877887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51811
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XK, also named as XKR1 and XRG1, is responsible for the Kx blood group system. XK forms a functional complex with the Kell glycoprotein. It represents a crucial factor for the maintenance of normal muscle structure and function.

实验方案

Product Specific Protocols
WB protocol for XK antibody 12830-1-APDownload protocol
IHC protocol for XK antibody 12830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols