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Conjugate
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XPA Polyclonal antibody, PBS Only

XPA Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16462-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy XPA Ready-To-Use IHC Kit. XPA primary antibody included.

产品信息

16462-1-PBS targets XPA in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen XPA fusion protein Ag9536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name xeroderma pigmentosum, complementation group A
Synonyms
Calculated Molecular Weight374aa,42 kDa; 273aa,31 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014965
Gene Symbol XPA
Gene ID (NCBI) 7507
RRIDAB_2878261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23025
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).