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验证数据展示

  • WB analysis using 15655-1-AP

    WB analysis using 15655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 15655-1-AP

    IP experiment of PC-3 using 15655-1-AP

    IP result of anti-XPNPEP3 (IP:15655-1-AP, 4ug; Detection:15655-1-AP 1:400) with PC-3 cells lysate 2000ug.

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XPNPEP3 Polyclonal antibody

XPNPEP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15655-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver
Positive IP detected inPC-3 cells
Positive IHC detected inmouse liver tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15655-1-AP targets XPNPEP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 XPNPEP3 fusion protein Ag8007 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X-prolyl aminopeptidase (aminopeptidase P) 3, putative
别名 X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC004989
基因名称 XPNPEP3
Gene ID (NCBI) 63929
RRIDAB_2288500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQH7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356).

实验方案

Product Specific Protocols
WB protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IHC protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IP protocol for XPNPEP3 antibody 15655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols