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XPNPEP3 Polyclonal antibody

XPNPEP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15655-1-AP

Print datasheet

Synonyms

X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3

验证数据展示

  • WB analysis using 15655-1-AP

    WB analysis using 15655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 15655-1-AP

    IP experiment of PC-3 using 15655-1-AP

    IP result of anti-XPNPEP3 (IP:15655-1-AP, 4ug; Detection:15655-1-AP 1:400) with PC-3 cells lysate 2000ug.

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver
Positive IP detected inPC-3 cells
Positive IHC detected inmouse liver tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15655-1-AP targets XPNPEP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen XPNPEP3 fusion protein Ag8007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-prolyl aminopeptidase (aminopeptidase P) 3, putative
Synonyms X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC004989
Gene Symbol XPNPEP3
Gene ID (NCBI) 63929
RRIDAB_2288500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQH7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356).

实验方案

Product Specific Protocols
WB protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IHC protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IP protocol for XPNPEP3 antibody 15655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols