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验证数据展示

  • WB analysis using 28628-1-AP

    WB analysis using 28628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28628-1-AP (XPO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 28628-1-AP

    IHC staining of mouse testis using 28628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28628-1-AP (XPO5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28628-1-AP

    IHC staining of mouse testis using 28628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28628-1-AP (XPO5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XPO5 Polyclonal antibody

XPO5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28628-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (1)

应用

WB, IHC, ELISA

别名

Exp5, exportin 5, FLJ14239, FLJ32057, FLJ45606, KIAA1291, Ran binding protein 21, RANBP21, XPO5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28628-1-AP targets XPO5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse
文献引用反应性human
免疫原 XPO5 fusion protein Ag24217 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exportin 5
别名 Exp5, exportin 5, FLJ14239, FLJ32057, FLJ45606, KIAA1291, Ran binding protein 21, RANBP21, XPO5
计算分子量 136 kDa
观测分子量136 kDa
GenBank蛋白编号BC000129
基因名称 XPO5
Gene ID (NCBI) 57510
RRIDAB_2881184
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XPO5 antibody 28628-1-APDownload protocol
IHC protocol for XPO5 antibody 28628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5

Authors - Xingyuan Chen
View Article

Ecotoxicol Environ Saf

Soybean isoflavones protect dopaminergic neurons from atrazine damage by inhibiting VPS13A to increase autophagy

Authors - Peng Li
View Article