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XPO5 Polyclonal antibody

XPO5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28628-1-AP

Print datasheet

Synonyms

Exp5, exportin 5, FLJ14239, FLJ32057, FLJ45606, KIAA1291, Ran binding protein 21, RANBP21, XPO5

验证数据展示

  • WB analysis using 28628-1-AP

    WB analysis using 28628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28628-1-AP (XPO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 28628-1-AP

    IHC staining of mouse testis using 28628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28628-1-AP (XPO5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28628-1-AP

    IHC staining of mouse testis using 28628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28628-1-AP (XPO5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28628-1-AP targets XPO5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen XPO5 fusion protein Ag24217 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exportin 5
Synonyms Exp5, exportin 5, FLJ14239, FLJ32057, FLJ45606, KIAA1291, Ran binding protein 21, RANBP21, XPO5
Calculated Molecular Weight 136 kDa
Observed Molecular Weight136 kDa
GenBank Accession NumberBC000129
Gene Symbol XPO5
Gene ID (NCBI) 57510
RRIDAB_2881184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XPO5 antibody 28628-1-APDownload protocol
IHC protocol for XPO5 antibody 28628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5

Authors - Xingyuan Chen
View Article