验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11408-1-AP targets XPO6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, drosophila |
| 免疫原 | XPO6 fusion protein Ag1898 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exportin 6 |
| 别名 | EXP6, exportin 6, KIAA0370, Ran binding protein 20, RANBP20, XPO6 |
| 计算分子量 | 1125 aa, 129 kDa |
| 观测分子量 | 128 kDa |
| GenBank蛋白编号 | BC014071 |
| 基因名称 | XPO6 |
| Gene ID (NCBI) | 23214 |
| RRID | AB_2241610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96QU8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XPO6 antibody 11408-1-AP | Download protocol |
| IHC protocol for XPO6 antibody 11408-1-AP | Download protocol |
| IP protocol for XPO6 antibody 11408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. | ||
Proc Natl Acad Sci U S A Active maintenance of nuclear actin by importin 9 supports transcription. | ||
Cancer Res Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer. | ||
J Virol The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.
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