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验证数据展示

  • WB analysis using 84902-1-RR

    WB analysis using 84902-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84902-1-RR

    Affinity and Kinetic Characterization of 84902-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

XPR1 Recombinant antibody

XPR1 Recombinant Antibody for WB, ELISA
Cat No. 84902-1-RR

Print datasheet

CloneNo. 241788A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog, Phosphate exporter SLC53A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84902-1-RR targets XPR1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen XPR1 fusion protein Ag5373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name xenotropic and polytropic retrovirus receptor
Synonyms SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog, Phosphate exporter SLC53A1
Calculated Molecular Weight 696 aa, 82 kDa
Observed Molecular Weight74 kDa
GenBank Accession NumberBC041142
Gene Symbol XPR1
Gene ID (NCBI) 9213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBH6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.

实验方案

Product Specific Protocols
WB protocol for XPR1 antibody 84902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols