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验证数据展示

  • WB analysis using 84902-1-RR

    WB analysis using 84902-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84902-1-RR

    Affinity and Kinetic Characterization of 84902-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

XPR1 Recombinant antibody

XPR1 Recombinant Antibody for WB, ELISA
Cat No. 84902-1-RR

产品说明书

CloneNo. 241788A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog, Phosphate exporter SLC53A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84902-1-RR targets XPR1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 XPR1 fusion protein Ag5373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 xenotropic and polytropic retrovirus receptor
别名 SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog, Phosphate exporter SLC53A1
计算分子量 696 aa, 82 kDa
观测分子量74 kDa
GenBank蛋白编号BC041142
基因名称 XPR1
Gene ID (NCBI) 9213
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBH6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.

实验方案

Product Specific Protocols
WB protocol for XPR1 antibody 84902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols