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验证数据展示

  • WB analysis of MCF-7 using 18494-1-AP

    WB analysis of MCF-7 using 18494-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18494-1-AP (XRCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 18494-1-AP

    IF Staining of MCF-7 using 18494-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 18494-1-AP (XRCC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

XRCC3 Polyclonal antibody

XRCC3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18494-1-AP

Print datasheet

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

X-ray repair cross-complementing protein 3, CMM6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

18494-1-AP targets XRCC3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen XRCC3 fusion protein Ag13317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 3
Synonyms X-ray repair cross-complementing protein 3, CMM6
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC011725
Gene Symbol XRCC3
Gene ID (NCBI) 7517
RRIDAB_2878546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XRCC3 antibody 18494-1-APDownload protocol
IF protocol for XRCC3 antibody 18494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis

Authors - Yunshang Chen
View Article
humanWB

Int J Biochem Cell Biol

MiroRNA-127-3p targets XRCC3 to enhance the chemosensitivity of esophageal cancer cells to a novel phenanthroline-dione derivative.

Authors - Ni Zhen
View Article
humanWB

Nat Struct Mol Biol

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development

Authors - Nazareno Bona
View Article