验证数据展示
经过测试的应用
Positive WB detected in | NCI-H1299 cells, HT-29 cells, Caco-2 cells, HT-1080 cells, HeLa cells, MCF-7 cells, COLO 320 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | mouse testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67222-1-Ig targets XRCC3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
经测试反应性 | Human, Mouse |
免疫原 | XRCC3 fusion protein Ag13317 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | X-ray repair complementing defective repair in Chinese hamster cells 3 |
别名 | DNA repair protein XRCC3, XRCC3 |
计算分子量 | 38 kDa |
观测分子量 | 38 kDa |
GenBank蛋白编号 | BC011725 |
基因名称 | XRCC3 |
Gene ID (NCBI) | 7517 |
RRID | AB_2882513 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O43542 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for XRCC3 antibody 67222-1-Ig | Download protocol |
IHC protocol for XRCC3 antibody 67222-1-Ig | Download protocol |
IF protocol for XRCC3 antibody 67222-1-Ig | Download protocol |
IP protocol for XRCC3 antibody 67222-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |