验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, COLO 320 cells, MCF-7 cells, A431 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy XRCC4 Ready-To-Use IHC Kit. XRCC4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66621-1-Ig targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | XRCC4 fusion protein Ag8807 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | X-ray repair complementing defective repair in Chinese hamster cells 4 |
| 别名 | DNA repair protein XRCC4, XRCC4 |
| 计算分子量 | 310 aa, 35 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC005259 |
| 基因名称 | XRCC4 |
| Gene ID (NCBI) | 7518 |
| RRID | AB_2881981 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13426 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XRCC4 antibody 66621-1-Ig | Download protocol |
| IHC protocol for XRCC4 antibody 66621-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
