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  • Featured Product
  • KD/KO Validated

XRCC4 Monoclonal antibody

XRCC4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E5D2

Cat no : 66621-1-Ig

Print datasheet

Synonyms

DNA repair protein XRCC4, XRCC4

验证数据展示

  • WB analysis using 66621-1-Ig

    WB analysis using 66621-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66621-1-Ig

    WB analysis of HEK-293 using 66621-1-Ig

    WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 66621-1-Ig

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with XRCC4 antibody included
  • IHC staining of human colon cancer using 66621-1-Ig

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with XRCC4 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, COLO 320 cells, MCF-7 cells, A431 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy XRCC4 Ready-To-Use IHC Kit. XRCC4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66621-1-Ig targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen XRCC4 fusion protein Ag8807 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Synonyms DNA repair protein XRCC4, XRCC4
Calculated Molecular Weight 310 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005259
Gene Symbol XRCC4
Gene ID (NCBI) 7518
RRIDAB_2881981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13426
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XRCC4 antibody 66621-1-IgDownload protocol
IHC protocol for XRCC4 antibody 66621-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
  • KD Validated
View Article