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验证数据展示

  • WB analysis using 66546-1-Ig (same clone as 66546-1-PBS)

    WB analysis using 66546-1-Ig (same clone as 66546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66546-1-Ig (XRCC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP result of anti-XRCC5/Ku80 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:20000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC5 antibody included
  • IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XRCC5 antibody (66546-1-Ig, Clone: 2G5E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP result of anti-XRCC5/Ku80 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:40000) with HeLa cells lysate 640 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66546-1-Ig (XRCC5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

XRCC5/Ku80 Monoclonal antibody, PBS Only

XRCC5/Ku80 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66546-1-PBS

产品说明书

CloneNo. 2G5E7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KU80, XRCC5, 2G5E7, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

产品信息

66546-1-PBS targets XRCC5/Ku80 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 XRCC5/Ku80 fusion protein Ag9512 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
别名 KU80, XRCC5, 2G5E7, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit
计算分子量 732 aa, 83 kDa
观测分子量 80-83 kDa
GenBank蛋白编号BC019027
基因名称 XRCC5
Gene ID (NCBI) 7520
RRIDAB_2881908
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13010
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.