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XRCC5 Recombinant antibody, PBS Only (Detector)

XRCC5 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240301A1

Cat no : 80325-2-PBS

Print datasheet

Synonyms

KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit

验证数据展示

  • Sandwich ELISA standard curve of MP00384-2

    Sandwich ELISA standard curve of MP00384-2

    Sandwich ELISA standard curve of MP00384-2, Human XRCC5 Recombinant Matched Antibody Pair - PBS only. 80325-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9454. 80325-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Sample test of MP00384-2

    Sample test of MP00384-2

    HEK-293, Hela, K-562 and A549 cell lysates were measured. The human XRCC5 concentration of detected samples was determined to be 27,396.5 pg/mL (based on a 1.20 mg/mL extract load) in HEK-293 cell lysate, 59,588.9 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate, 74,969.1 pg/mL (based on a 2.10 mg/mL extract load) in K-562 cell lysate and 71,822.7 pg/mL (based on a 1.00 mg/mL extract load) in A549 cell lysate.

  • Cytometric bead array standard curve of MP00384-2

    Cytometric bead array standard curve of MP00384-2

    Cytometric bead array standard curve of MP00384-2, XRCC5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80325-3-PBS. Detection antibody: 80325-2-PBS. Standard: Ag9454. Range: 0.625-80 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

产品信息

80325-2-PBS targets XRCC5 as part of a matched antibody pair:

MP00384-2: 80325-3-PBS capture and 80325-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen XRCC5 fusion protein Ag9454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Synonyms KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit
Calculated Molecular Weight 732 aa, 83 kDa
GenBank Accession NumberBC019027
Gene Symbol XRCC5
Gene ID (NCBI) 7520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13010
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C