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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66852-1-Ig (same clone as 66852-1-PBS)

    WB analysis using 66852-1-Ig (same clone as 66852-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRN2 antibody included
  • IF Staining of MCF-7 using 66852-1-Ig (same clone as 66852-1-PBS)

    IF Staining of MCF-7 using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using XRN2 antibody (66852-1-Ig, Clone: 2C3E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66852-1-Ig (same clone as 66852-1-PBS)

    FC experiment of HepG2 using 66852-1-Ig (same clone as 66852-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRN2 (66852-1-Ig, Clone:2C3E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

XRN2 Monoclonal antibody, PBS Only

XRN2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66852-1-PBS

产品说明书

CloneNo. 2C3E3

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.

产品信息

66852-1-PBS targets XRN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 XRN2 fusion protein Ag27927 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 5'-3' exoribonuclease 2
别名 DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2
计算分子量 104 kDa
观测分子量 109 kDa
GenBank蛋白编号BC006417
基因名称 XRN2
Gene ID (NCBI) 22803
RRIDAB_2882192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0D6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.