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XRN2 Monoclonal antibody, PBS Only

XRN2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C3E3

Cat no : 66852-1-PBS

Print datasheet

Synonyms

DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2

验证数据展示

  • WB analysis using 66852-1-Ig (same clone as 66852-1-PBS)

    WB analysis using 66852-1-Ig (same clone as 66852-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRN2 antibody included
  • IF Staining of MCF-7 using 66852-1-Ig (same clone as 66852-1-PBS)

    IF Staining of MCF-7 using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using XRN2 antibody (66852-1-Ig, Clone: 2C3E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66852-1-Ig (same clone as 66852-1-PBS)

    FC experiment of HepG2 using 66852-1-Ig (same clone as 66852-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRN2 (66852-1-Ig, Clone:2C3E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.

产品信息

66852-1-PBS targets XRN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen XRN2 fusion protein Ag27927 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 5'-3' exoribonuclease 2
Synonyms DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC006417
Gene Symbol XRN2
Gene ID (NCBI) 22803
RRIDAB_2882192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0D6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.